| 质粒名称: | PGM2L1 gRNA (BRDN0001149301) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 77145 |
| 存储实验室: | John Doench, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | lentiGuide-Puro |
| 载体出品公司: | Zhang Lab (Addgene plasmid # 52963) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PGM2L1,PGM2L1,NM_173582.4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | hU6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | U6-F |
| 3' 测序引物 | pS2-R |
|
文献引用方法 |
|
| 材料和方法部分: | PGM2L1 gRNA (BRDN0001149301) was a gift from John Doench & David Root (Addgene plasmid # 77145 ; http://n2t.net/addgene:77145 ; RRID:Addgene_77145) |
| 参考文献部分: | Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. 10.1038/nbt.3437 PubMed 26780180 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS PGM2L1 gRNA (BRDN0001149301) 8323 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 3rd generation lentiviral gRNA plasmid targeting human PGM2L1.
ACCESSION .
VERSION .
KEYWORDS PGM2L1 gRNA (BRDN0001149301)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8323)
AUTHORS Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF,
Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J,
Root DE
TITLE Optimized sgRNA design to maximize activity and minimize off-target
effects of CRISPR-Cas9.
JOURNAL Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
PUBMED 26780180
REFERENCE 2 (bases 1 to 8323)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8323
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 87..1265
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 318..1256
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1213..1233
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 1278..1877
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1278..1297)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1774..1794
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 1893..2481
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1946..1966)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2364..2375)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 2553..2786
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2864..2985
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2901..2920)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2955..2974
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3025..3160
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3087..3106
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3180..3199)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3181..3199)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3209..3226)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3209..3225)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3218..3240)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3367..3822
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3454..3473)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3664..3685
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3848..3952
/gene="bla"
/label=AmpR promoter
CDS 3953..4813
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4171..4190)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4984..5572
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5473..5492
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5726..5743
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5860..5881
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5896..5926
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5934..5950
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5939..5961
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5958..5974
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5958..5974
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 5993..6013
/label=T3
/note="T3 promoter, forward primer"
promoter 5995..6013
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6041..6267
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6268..6448
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6495..6620
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7113..7346
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 7753..7993
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 7753..7773
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 7924..7943
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 8023..8098
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 8154..8271
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
1261 cgtgacgtac ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
1321 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
1381 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
1441 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
1501 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
1561 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
1621 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc
1681 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
1741 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
1801 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
1861 gcaagcccgg tgcctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
1921 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
1981 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
2041 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
2101 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
2161 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
2221 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
2281 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
2341 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
2401 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
2461 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
2521 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
2581 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
2641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
2701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
2761 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
2821 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
2881 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
2941 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
3001 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
3061 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
3121 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
3181 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
3241 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
3301 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
3361 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
3421 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
3481 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
3541 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
3601 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
3661 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
3721 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
3781 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
3841 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3901 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
3961 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
4021 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
4081 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
4141 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
4201 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
4261 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
4321 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
4381 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
4441 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
4501 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
4561 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
4621 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
4681 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
4741 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
4801 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
4861 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
4921 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
4981 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
5041 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
5101 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
5161 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
5221 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
5281 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
5341 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
5401 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
5461 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
5521 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
5581 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
5641 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
5701 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
5761 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
5821 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
5881 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
5941 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
6001 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
6061 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
6121 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
6181 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
6241 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
6301 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
6361 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
6421 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
6481 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
6541 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
6601 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
6661 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
6721 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
6781 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
6841 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
6901 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
6961 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
7021 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
7081 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
7141 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
7201 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
7261 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
7321 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
7381 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
7441 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
7501 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
7561 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
7621 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
7681 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
7741 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
7801 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
7861 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
7921 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
7981 ttgtggaaag gacgaaacac cgaggtaccc accttatccc aggttttaga gctagaaata
8041 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
8101 ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
8161 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
8221 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
8281 tacagggaca gcagagatcc actttggcgc cggctcgagg ggg
//
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